Aronov, Pavel A. and Hall, Laura M. and Dettmer, Katja and Stephensen, Charles B. and Hammock, Bruce D. (2008) Metabolic profiling of major vitamin D metabolites using Diels-Alder derivatization and ultra-performance liquid chromatography-tandem mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 391 (5). pp. 1917-1930. ISSN 1618-2642,
Full text not available from this repository. (Request a copy)Abstract
Biologically active forms of vitamin D are important analytical targets in both research and clinical practice. The current technology is such that each of the vitamin D metabolites is usually analyzed by individual assay. However, current LC-MS technologies allow the simultaneous metabolic profiling of entire biochemical pathways. The impediment to the metabolic profiling of vitamin D metabolites is the low level of 1 alpha,25-dihydroxyvitamin D-3 in human serum (15-60 pg/mL). Here, we demonstrate that liquid-liquid or solid-phase extraction of vitamin D metabolites in combination with Diels-Alder derivatization with the commercially available reagent 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) followed by ultra-performance liquid chromatography (UPLC)-electrospray/tandem mass spectrometry analysis provides rapid and simultaneous quantification of 1 alpha,25-dihydroxyvitamin D-3, 1 alpha,25-dihydroxyvitamin D-2, 24R,25-dihydroxyvitamin D-3, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 in 0.5 mL human serum at a lower limit of quantification of 25 pg/mL. Precision ranged from 1.6-4.8 % and 5-16 % for 25-hydroxyvitamin D-3 and 1 alpha,25-dihydroxyvitamin D-3, respectively, using solid-phase extraction.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AVIUM-INTRACELLULARE INFECTION; COOKSON-TYPE REAGENT; 25-HYDROXYVITAMIN D-3; HUMAN PLASMA; HYPERCALCEMIA; PATIENT; 1,25-DIHYDROXYVITAMIN-D; AIDS; 1-ALPHA,25-DIHYDROXYVITAMIN-D-3; PERSPECTIVE; 1 alpha,25-dihydroxyvitamin D-3; 25-hydroxyvitamin D-3; 24R,25-dihydroxyvitamin D-3; UPLC; LC-MS; metabolic profiling; derivatization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Oct 2020 09:10 |
| Last Modified: | 28 Oct 2020 09:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30632 |
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