Kocincova, Anna S. and Nagl, Stefan and Arain, Sarina and Krause, Christian and Borisov, Sergey M. and Arnold, Matthias and Wolfbeis, Otto S. (2008) Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH. BIOTECHNOLOGY AND BIOENGINEERING, 100 (3). pp. 430-438. ISSN 0006-3592,
Full text not available from this repository. (Request a copy)Abstract
Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase-domain fluorescence lifetime-based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24-well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell-based high-throughput screening applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAMMALIAN-CELL CULTURE; MICROTITER PLATES; MICROBIOREACTOR; BIOREACTORS; PERFORMANCE; PARAMETERS; OPTODES; DESIGN; SYSTEM; oxygen sensing; pH sensing; microbioreactor; bioprocess; dual sensor; fluorescence |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Oct 2020 06:50 |
| Last Modified: | 29 Oct 2020 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30729 |
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