Cigler, Petr and Kral, Vladimir and Kozisek, Milan and Konvalinka, Jan and Mirsky, Vladimir M. (2008) Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization? COLLOIDS AND SURFACES B-BIOINTERFACES, 64 (1). pp. 145-149. ISSN 0927-7765,
Full text not available from this repository. (Request a copy)Abstract
Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELF-ASSEMBLED MONOLAYERS; OPTICAL BIOSENSOR TECHNOLOGY; RETROVIRAL PROTEASES; INTERACTION KINETICS; INHIBITORS; RESISTANCE; SURFACES; PROTEINS; GOLD; BINDING; HIV protease; protein adsorption; protein-resistant surfaces; self-assembled monolayer; surface plasmon resonance (SPR) |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Oct 2020 07:57 |
| Last Modified: | 29 Oct 2020 07:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30730 |
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