Elkins, James G. and Podar, Mircea and Graham, David E. and Makarova, Kira S. and Wolf, Yuri and Randau, Lennart and Hedlund, Brian P. and Brochier-Armanet, Celine and Kunin, Victor and Anderson, Iain and Lapidus, Alla and Goltsman, Eugene and Barry, Kerrie and Koonin, Eugene V. and Hugenholtz, Phil and Kyrpides, Nikos and Wanner, Gerhard and Richardson, Paul and Keller, Martin and Stetter, Karl O. (2008) A korarchaeal genome reveals insights into the evolution of the Archaea. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105 (23). pp. 8102-8107. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
The candidate division Korarchaeota comprises a group of uncultivated microorganisms that, by their small subunit rRNA phylogeny, may have diverged early from the major archaeal phyla Crenarchaeota and Euryarchaeota. Here, we report the initial characterization of a member of the Korarchaeota with the proposed name, "Candidatus Korarchaeum cryptofilum," which exhibits an ultrathin filamentous morphology. To investigate possible ancestral relationships between deep-branching Korarchaeota and other phyla, we used whole-genome shotgun sequencing to construct a complete composite korarchaeal genome from enriched cells. The genome was assembled into a single contig 1.59 Mb in length with a G + C content of 49%. Of the 1,617 predicted protein-coding genes, 1,382,(85%) could be assigned to a revised set of archaeal Clusters of Orthologous Groups (COGs). The predicted gene functions suggest that the organism relies on a simple mode of peptide fermentation for carbon and energy and lacks the ability to synthesize de novo purines, CoA, and several other cofactors. Phylogenetic analyses based on conserved single genes and concatenated protein sequences positioned the korarchaeote as a deep archaeal lineage with an apparent affinity to the Crenarchaeota. However, the predicted gene content revealed that several conserved cellular systems, such as cell division, DNA replication, and tRNA maturation, resemble the counterparts in the Euryarchaeota. In light of the known composition of archaeal genomes, the Korarchaeota might have retained a set of cellular features that represents the ancestral archaeal form.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | YELLOWSTONE-NATIONAL-PARK; TRANSFER-RNA GENES; PYROCOCCUS-FURIOSUS; FERREDOXIN OXIDOREDUCTASE; MICROBIAL DIVERSITY; SPECIES COMPOSITION; CELL-DIVISION; SEQUENCE; BACTERIA; SEA; microbial cultivation; genomics; hyperthermophiles; Korarchaeota; phylogeny |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Oct 2020 10:08 |
| Last Modified: | 29 Oct 2020 10:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30742 |
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