Leutenegger, Marcel and Lasser, Theo and Sinner, Eva-Kathrin and Robelek, Rudolf (2008) Imaging of G protein-coupled receptors in solid-supported planar lipid membranes. BIOINTERPHASES, 3 (2). FA136-FA145. ISSN 1559-4106,
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This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a novel approach for the functional incorporation of membrane proteins, i.e., by their cell-free expression and in vitro reconstitution in the presence of tBLMs. By the combination of the corresponding coding DNA with the protein synthesis machinery of a cell-extract (in vitro transcription and translation), the authors observe spontaneous and vectorial insertions of an interesting example of the GPCR family into a tethered bimolecular lipid membrane: the olfactory receptor OR5. After synthesis, imaging of the surface area is performed and the resulting signals are analyzed in order to resolve quantity and lateral mobilities. Ligand-independent aggregation behavior of the GPCRs and quantitative analysis of the fluorescent signals are presented in this work.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; CORRELATION SPECTROSCOPY; FLUORESCENCE CORRELATION; ACTIVATION; MODEL; biological techniques; biomembranes; fluorescence spectroscopy; lipid bilayers; molecular biophysics; proteins |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Oct 2020 12:03 |
| Last Modified: | 29 Oct 2020 12:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30767 |
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