Henquet, Maurice and Lehle, Ludwig and Schreuder, Marielle and Rouwendal, Gerard and Molthoff, Jos and Helsper, Johannes and van der Krol, Sander and Bosch, Dirk (2008) Identification of the gene encoding the alpha 1,3-mannosyltransferase (ALG3) in Arabidopsis and characterization of downstream N-glycan processing. PLANT CELL, 20 (6). pp. 1652-1664. ISSN 1040-4651,
Full text not available from this repository. (Request a copy)Abstract
Glycosyltransferases are involved in the biosynthesis of lipid-linked N-glycans. Here, we identify and characterize a mannosyltransferase gene from Arabidopsis thaliana, which is the functional homolog of the ALG3 (Dol-P-Man: Man(5)GlcNAc(2)-PP-Dol alpha 1,3-mannosyl transferase) gene in yeast. The At ALG3 protein can complement a Delta alg3 yeast mutant and is localized to the endoplasmic reticulum in yeast and in plants. A homozygous T-DNA insertion mutant, alg3-2, was identified in Arabidopsis with residual levels of wild-type ALG3, derived from incidental splicing of the 11th intron carrying the T-DNAs. N- glycan analysis of alg3-2 and alg3-2 in the complex-glycan-less mutant background, which lacks N- acetylglucosaminyl-transferase I activity, reveals that when ALG3 activity is strongly reduced, almost all N-glycans transferred to proteins are aberrant, indicating that the Arabidopsis oligosaccharide transferase complex is remarkably substrate tolerant. In alg3-2 plants, the aberrant glycans on glycoproteins are recognized by endogenous mannosidase I and N- acetylglucosaminyltransferase I and efficiently processed into complex-type glycans. Although no high-mannose-type glycoproteins are detected in alg3-2 plants, these plants do not show a growth phenotype under normal growth conditions. However, the glycosylation abnormalities result in activation of marker genes diagnostic of the unfolded protein response.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ASPARAGINE-LINKED OLIGOSACCHARIDES; DEFICIENT GLYCOPROTEIN SYNDROME; UNFOLDED PROTEIN RESPONSE; YEAST MUTANTS DEFICIENT; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; CONGENITAL DISORDER; OLIGOSACCHARYLTRANSFERASE COMPLEX; ACETYLGLUCOSAMINYLTRANSFERASE-I; GLYCOSYLATION PATHWAY; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Nov 2020 08:23 |
| Last Modified: | 02 Nov 2020 08:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30861 |
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