Fuechsl, Andrea M. and Reber, Stefan O. (2016) Chronic Psychosocial Stress and Negative Feedback Inhibition: Enhanced Hippocampal Glucocorticoid Signaling despite Lower Cytoplasmic GR Expression. PLOS ONE, 11 (4): e0153164. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Chronic subordinate colony housing (CSC), a pre-clinically validated mouse model for chronic psychosocial stress, results in increased basal and acute stress-induced plasma adrenocorticotropic hormone (ACTH) levels. We assessed CSC effects on hippocampal glucocorticoid (GC) receptor (GR), mineralocorticoid receptor (MR), and FK506 binding protein (FKBP51) expression, acute heterotypic stressor-induced GR translocation, as well as GC effects on gene expression and cell viability in isolated hippocampal cells. CSC mice showed decreased GR mRNA and cytoplasmic protein levels compared with single-housed control (SHC) mice. Basal and acute stress-induced nuclear GR protein expression were comparable between CSC and SHC mice, as were MR and FKBP51 mRNA and/or cytoplasmic protein levels. In vitro the effect of corticosterone (CORT) on hippocampal cell viability and gene transcription was more pronounced in CSC versus SHC mice. In summary, CSC mice show an, if at all, increased hippocampal GC signaling capacity despite lower cytoplasmic GR protein expression, making negative feedback deficits in the hippocampus unlikely to contribute to the increased ACTH drive following CSC.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA EXPRESSION; PITUITARY-ADRENAL AXIS; RAT-BRAIN; MALE-MICE; ALZHEIMERS-DISEASE; GENE-EXPRESSION; HPA AXIS; CORTICOSTERONE; RECEPTOR; MECHANISMS; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2019 08:15 |
| Last Modified: | 05 Apr 2019 08:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3089 |
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