Boeger, Hinrich and Griesenbeck, Joachim and Kornberg, Roger D. (2008) Nucleosome retention and the stochastic nature of promoter chromatin remodeling for transcription. CELL, 133 (4). pp. 716-726. ISSN 0092-8674, 1097-4172
Full text not available from this repository. (Request a copy)Abstract
The rate-limiting step of transcriptional activation in eukaryotes, and thus the critical point for gene regulation, is unknown. Combining biochemical analyses of the chromatin transition at the transcriptionally induced PHO5 promoter in yeast with modeling based on a small number of simple assumptions, we demonstrate that random removal and reformation of promoter nucleosomes can account for stochastic and kinetic properties of PHO5 expression. Our analysis suggests that the disassembly of promoter nucleosomes is rate limiting for PHO5 expression, and supports a model for the underlying mechanism of promoter chromatin remodeling, which appears to conserve a single nucleosome on the promoter at all times.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHO5 PROMOTER; HISTONE ACETYLATION; DNA TRANSLOCATION; PH05 PROMOTER; IN-VIVO; YEAST; SWI/SNF; ACTIVATION; COMPLEX; REPLICATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 05:47 |
| Last Modified: | 03 Nov 2020 05:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30898 |
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