Jha, Kula N. and Shumilin, Igor A. and Digilio, Laura C. and Chertihin, Olga and Zheng, Heping and Schmitz, Gerd and Visconti, Pablo E. and Flickinger, Charles J. and Minor, Wladek and Herr, John C. (2008) Biochemical and structural characterization of apolipoprotein A-I binding protein, a novel phosphoprotein with a potential role in sperm capacitation. ENDOCRINOLOGY, 149 (5). pp. 2108-2120. ISSN 0013-7227, 1945-7170
Full text not available from this repository. (Request a copy)Abstract
The physiological changes that sperm undergo in the female reproductive tract rendering them fertilization-competent constitute the phenomenon of capacitation. Cholesterol efflux from the sperm surface and protein kinase A(PKA)-dependent phosphorylation play major regulatory roles in capacitation, but the link between these two phenomena is unknown. We report that apolipoprotein A-I binding protein (AI-BP) is phosphorylated downstream to PKA activation, localizes to both sperm head and tail domains, and is released from the sperm into the media during in vitro capacitation. AI-BP interacts with apolipoprotein A-I, the component of high-density lipoprotein involved in cholesterol transport. The crystal structure demonstrates that the subunit of the AI-BP homodimer has a Rossmann-like fold. The protein surface has a large two compartment cavity lined with conserved residues. This cavity is likely to constitute an active site, suggesting that AI-BP functions as an enzyme. The presence of AI-BP in sperm, its phosphorylation by PKA, and its release during capacitation suggest that AI-BP plays an important role in capacitation possibly providing a link between protein phosphorylation and cholesterol efflux.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FAMILIAL COMBINED HYPERLIPIDEMIA; SOLUBLE ADENYLYL-CYCLASE; QUANTITATIVE TRAIT LOCUS; X-RAY-DIFFRACTION; TYROSINE PHOSPHORYLATION; CHOLESTEROL EFFLUX; HUMAN-SPERMATOZOA; MOUSE SPERM; MAMMALIAN SPERMATOZOA; CHROMOSOME 1Q21-Q23; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 08:12 |
| Last Modified: | 03 Nov 2020 08:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30933 |
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