Merz, Katharina and Hondele, Maria and Goetze, Hannah and Gmelch, Katharina and Stoeckl, Ulrike and Griesenbeck, Joachim (2008) Actively transcribed rRNA genes in S-cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. GENES & DEVELOPMENT, 22 (9). pp. 1190-1204. ISSN 0890-9369, 1549-5477
Full text not available from this repository. (Request a copy)Abstract
Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE-I TRANSCRIPTION; REPLICATION FORK BLOCKING; FOB1 PROTEIN; RDNA TRANSCRIPTION; YEAST RDNA; PROMOTER; DNA; COMPLEX; UPSTREAM; INITIATION; chromatin; transcription; ribosomal DNA |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 09:21 |
| Last Modified: | 03 Nov 2020 09:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30945 |
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