Engel, Eva and Schraml, Ruediger and Maisch, Tim and Kobuch, Karin and Koenig, Burkhard and Szeimies, Rolf-Markus and Hillenkamp, Jost and Baeumler, Wolfgang and Vasold, Rudolf (2008) Light-induced decomposition of indocyanine green. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 49 (5). pp. 1777-1783. ISSN 0146-0404,
Full text not available from this repository. (Request a copy)Abstract
PURPOSE. To investigate the light-induced decomposition of indocyanine green (ICG) and to test the cytotoxicity of light-induced ICG decomposition products. METHODS. ICG in solution was irradiated with laser light, solar light, or surgical endolight. The light-induced decomposition of ICG was analyzed by high-performance liquid chromatography (HPLC) and mass spectrometry. Porcine retinal pigment epithelial (RPE) cells were incubated with the light-induced decomposition products of ICG, and cell viability was measured by trypan blue exclusion assay. RESULTS. Independent of the light source used, singlet oxygen (photodynamic type 2 reaction) is generated by ICG leading to dioxetanes by [2 + 2]-cycloaddition of singlet oxygen. These dioxetanes thermally decompose into several carbonyl compounds. The decomposition products were identified by mass spectrometry. The decomposition of ICG was inhibited by adding sodium azide, a quencher of singlet oxygen. Incubation with ICG decomposition products significantly reduced the viability of RPE cells in contrast to control cells. CONCLUSIONS. ICG is decomposed by light within a self-sensitized photo oxidation. The decomposition products reduce the viability of RPE cells in vitro. The toxic effects of decomposed ICG should be further investigated under in vivo conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERNAL LIMITING MEMBRANE; MACULAR HOLE SURGERY; RETINAL-PIGMENT EPITHELIUM; IDIOPATHIC EPIRETINAL MEMBRANE; CANCER CELLS; IN-VITRO; DYE; FLUORESCENCE; REMOVAL; OXYGEN; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde Medicine > Lehrstuhl für Dermatologie und Venerologie Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 09:36 |
| Last Modified: | 03 Nov 2020 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30954 |
Actions (login required)
 |
View Item |
