Matyash, Vitali and Liebisch, Gerhard and Kurzchalia, Teymuras V. and Shevchenko, Andrej and Schwudke, Dominik (2008) Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. JOURNAL OF LIPID RESEARCH, 49 (5). pp. 1137-1146. ISSN 0022-2275,
Full text not available from this repository. (Request a copy)Abstract
Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery of lipid species from analyzed cells, fluids, or tissues and is usually achieved by two-phase extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) extraction allows faster and cleaner lipid recovery and is well suited for automated shotgun profiling. Because of MTBE's low density, lipid-containing organic phase forms the upper layer during phase separation, which simplifies its collection and minimizes dripping losses. Nonextractable matrix forms a dense pellet at the bottom of the extraction tube and is easily removed by centrifugation. Rigorous testing demonstrated that the MTBE protocol delivers similar or better recoveries of species of most all major lipid classes compared with the "gold-standard" Folch or Bligh and Dyer recipes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; DATA-DEPENDENT ACQUISITION; ELECTROSPRAY-IONIZATION; QUANTITATIVE-ANALYSIS; MULTIPLE PRECURSOR; SHOTGUN LIPIDOMICS; ESCHERICHIA-COLI; ESI-MS/MS; QUANTIFICATION; PURIFICATION; mass spectrometry; sample preparation; direct infusion; nano-ESI-MS |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 10:11 |
| Last Modified: | 03 Nov 2020 10:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30964 |
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