Dreier, Rita and Opolka, Alfred and Grifka, Joachim and Bruckner, Peter and Graessel, Susanne (2008) Collagen IX-deficiency seriously compromises growth cartilage development in mice. MATRIX BIOLOGY, 27 (4). pp. 319-329. ISSN 0945-053X,
Full text not available from this repository. (Request a copy)Abstract
For a large part, skeletal development, growth, and repair occur by endochondral ossification which comprises an orderly sequence of consecutive steps of proliferation and late differentiation of chondrocytes. After vascular invasion into hypertrophic cartilage, the tissue is remodelled into bone. At all stages, the process is under tight environmental control exerted by a combination of regulators, including nutritional supply and signalling through growth factors, hormones, and cell-matrix-interactions. Therefore, genetic elimination of collagen IX, a stabilizing component of the periphery of thin cartilage fibrils, is expected to compromise extracellular matrix properties and, hence, the chondrocyte environment required for normal cartilage development and homeostasis. Here, we have shown that growth plate cartilage morphology is markedly disturbed in mice lacking collagen X Abnormalities were most prominent in late proliferative, pre-hypertrophic, and hypertrophic zones whereas restin and early proliferative zones were less affected. In central epiphyseal regions of long bones, newborn animals show grossly abnormal areas with strongly reduced cell numbers, irregular distribution of glycosaminoglycans in the extracellular matrix, and a profoundly disturbed columnar arrangement of chondrocytes with an irregular beta(1) integrin immunostaining. As a result, all long bones are shorter and broader in newborn Col9al-/- mice. Remarkably, these abnormalities are attenuated in adult mice, but the number of cells per area still is too low due to reduced cell proliferation. (c) 2008 Elsevier B.V./International Society of Matrix Biology. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIPLE EPIPHYSEAL DYSPLASIA; CHONDROCYTE PROLIFERATION; EXTRACELLULAR-MATRIX; CROSS-LINKING; FIBRILS; PROTEIN; CHONDROGENESIS; DECORIN; BINDING; PSEUDOACHONDROPLASIA; collagen IX; growth plate; hypertrophic zone; chondrocyte organization; morphology |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2020 10:54 |
| Last Modified: | 03 Nov 2020 10:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30974 |
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