Nemeth, Attila and Guibert, Sylvain and Tiwari, Vijay Kumar and Ohlsson, Rolf and Laengst, Gernot (2008) Epigenetic regulation of TTF-I-mediated promoter-terminator interactions of rRNA genes. EMBO JOURNAL, 27 (8). pp. 1255-1265. ISSN 0261-4189,
Full text not available from this repository. (Request a copy)Abstract
Ribosomal RNA synthesis is the eukaryotic cell's main transcriptional activity, but little is known about the chromatin domain organization and epigenetics of actively transcribed rRNA genes. Here, we show epigenetic and spatial organization of mouse rRNA genes at the molecular level. TTF-I-binding sites subdivide the rRNA transcription unit into functional chromatin domains and sharply delimit transcription factor occupancy. H2A. Z-containing nucleosomes occupy the spacer promoter next to a newly characterized TTF-I-binding site. The spacer and the promoter proximal TTF-I-binding sites demarcate the enhancer. DNA from both the enhancer and the coding region is hypomethylated in actively transcribed repeats. 3C analysis revealed an interaction between promoter and terminator regions, which brings the beginning and end of active rRNA genes into close contact. Reporter assays show that TTF-I mediates this interaction, thereby linking topology and epigenetic regulation of the rRNA genes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHROMOSOME CONFORMATION CAPTURE; REMODELING COMPLEX NORC; POLYMERASE-I; TRANSCRIPTION TERMINATION; NUCLEOLAR DOMINANCE; METHYLATION; BINDING; DNA; ORGANIZATION; INITIATION; chromatin; looping; nucleolus; transcription |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Nov 2020 07:39 |
| Last Modified: | 04 Nov 2020 07:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31036 |
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