Schuetz, Christian and Fleck, Martin and Mackensen, Andreas and Zoso, Alessia and Halbritter, Dagmar and Schneck, Jonathan P. and Oelke, Mathias (2008) Killer artificial antigen-presenting cells: a novel strategy to delete specific T cells. BLOOD, 111 (7). pp. 3546-3552. ISSN 0006-4971,
Full text not available from this repository. (Request a copy)Abstract
Several cell-based immunotherapy strategies have been developed to specifically modulate T cell-mediated immune responses. These methods frequently rely on the utilization of tolerogenic cell-based antigen-presenting cells (APCs). However, APCs are highly sensitive to cytotoxic T-cell responses, thus limiting their therapeutic capacity. Here, we describe a novel bead-based approach to modulate T-cell responses in an antigen-specific fashion. We have generated killer artificial APCs (kappa aAPCs) by coupling an apoptosis-inducing alpha-Fas (CD95) IgM mAb together with HLA-A(2) 19 molecules onto beads. These kappa aAPCs deplete targeted antigen-specific T cells in a Fas/Fas ligand (FasL)-dependent fashion. T-cell depletion in cocultures is rapidly initiated (30 minutes), dependent on the amount of kappa aAPCs and independent of activation-induced cell death (AICD). kappa aAPCs represent a novel technology that can control T cell-mediated immune responses, and therefore has potential for use in treatment of autoimmune diseases and allograft rejection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-MARROW-TRANSPLANTATION; EXPRESSING FAS LIGAND; DENDRITIC CELLS; IN-VIVO; CD95 LIGAND; MULTIPLE-SCLEROSIS; GRAFT-REJECTION; MURINE MODEL; INDUCTION; TOLERANCE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Nov 2020 13:44 |
| Last Modified: | 04 Nov 2020 13:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31075 |
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