Kroll, J. and Becker, K. F. and Kuphal, S. and Hein, R. and Hofstaedter, F. and Bosserhoff, A. K. (2008) Isolation of high quality protein samples from punches of formalin fixed and paraffin embedded tissue blocks. HISTOLOGY AND HISTOPATHOLOGY, 23 (4). pp. 391-395. ISSN 0213-3911,
Full text not available from this repository. (Request a copy)Abstract
In general, it is believed that the extraction of proteins from formalin-fixed paraffin embedded samples is not feasible. However, recently a new technique was developed, presenting the extraction of non-degraded, full length proteins from formalin fixed tissues, usable for western blotting and protein arrays. In the study presented here, we applied this technique to punch biopsies of formalin fixed tissues embedded in paraffin to reduce heterogeneity of the tissue represented in sections, and to ensure analysing mainly defined cellular material. Successful extraction was achieved even from very small samples (0.7 mm(3)). Additionally, we were able to detect highly glycosylated proteins and protein modification, such as phosphorylation. Interestingly, with this technique it is feasible to extract high quality proteins from 14 year old samples. In summary, the new technique makes a great pool of material now usable for molecular analysis with high throughput tools.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACTION; EXPRESSION; SECTIONS; MELANOMA; CANCER; formalin fixed tissues; protein extraction; western blotting; protein modification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Nov 2020 10:42 |
| Last Modified: | 04 Nov 2020 10:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31095 |
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