Ehlers, A. and Oker, E. and Bentink, S. and Lenze, D. and Stein, H. and Hummel, M. (2008) Histone acetylation and DNA demethylation of B cells result in a Hodgkin-like phenotype. LEUKEMIA, 22 (4). pp. 835-841. ISSN 0887-6924,
Full text not available from this repository. (Request a copy)Abstract
A unique feature of the tumor cells (Hodgkin/Reed-Sternberg (HRS)) of classical Hodgkin lymphoma (cHL) is the loss of their B-cell phenotype despite their B-cell origin. Several lines of evidence suggest that epigenomic events, especially promoter DNA methylation, are involved in this silencing of many B-cell-associated genes. Here, we show that DNA demethylation alone or in conjunction with histone acetylation is not able to reconstitute the B-cell-gene expression program in cultured HRS cells. Instead, combined DNA demethylation and histone acetylation of B-cell lines induce an almost complete extinction of their B-cell-expression program and a tremendous upregulation of numerous Hodgkin-characteristic genes, including key players such as Id2 known to be involved in the suppression of the B-cell phenotype. Since the upregulation of Hodgkin-characteristic genes and the extinction of the B-cell-expression program occurred simultaneously, epigenetic changes may also be responsible for the malignant transformation of cHL. The epigenetic upregulation of Hodgkin-characteristic genes thus plays-in addition to promoter DNA hypermethylation of B-cell-associated genes-a pivotal role for the reprogramming of HRS cells and explains why DNA demethylation alone is unable to reconstitute the B-cell-expression program in HRS cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REED-STERNBERG CELLS; GENE-EXPRESSION; IMMUNOGLOBULIN TRANSCRIPTION; FOLLICULAR LYMPHOMA; DISEASE; IDENTIFICATION; REARRANGEMENTS; INHIBITION; REPRESENT; PATTERN; methylation; acetylation; classical Hodgkin lymphoma; epigenetic |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 08:29 |
| Last Modified: | 05 Nov 2020 08:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31132 |
Actions (login required)
 |
View Item |
