Murawska, Magdalena and Kunert, Natascha and van Vugt, Joke and Laengst, Gernot and Kremmer, Elisabeth and Logie, Colin and Brehm, Alexander (2008) dCHD3, a novel ATP-Dependent chromatin remodeler associated with sites of active transcription. MOLECULAR AND CELLULAR BIOLOGY, 28 (8). pp. 2745-2757. ISSN 0270-7306,
Full text not available from this repository. (Request a copy)Abstract
ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChdI, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase 11 to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-II; YEAST SWI/SNF COMPLEX; HISTONE-H3 METHYLATION; DNA METHYLATION; BUDDING YEAST; CELL FATE; DROSOPHILA; PROTEIN; CHD1; CHROMODOMAINS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 08:37 |
| Last Modified: | 05 Nov 2020 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31137 |
Actions (login required)
 |
View Item |
