Heimer, Susanne and Liebisch, Gerhard and Le Lay, Soazig and Boettcher, Alfred and Wiesner, Philipp and Lindtner, Silke and Kurzchalia, Teymuras V. and Simons, Kai and Schmitz, Gerd (2008) Caveolin-1 deficiency alters plasma lipid and lipoprotein profiles in mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 367 (4). pp. 826-833. ISSN 0006-291X,
Full text not available from this repository. (Request a copy)Abstract
Caveolae are specialized membrane microdomains formed as the result of local accumulation of cholesterol, glycosphingolipids, and the structural protein caveolin-1 (Cav-1). To further elucidate the role of Cav-1 in lipid homeostasis in-vivo, we analyzed fasting and postprandial plasma from Cav-1 deficient mice on low or on high fat diet. In total plasma analysis, an increase in ceramide and hexosylceramide was observed. In cholesteryl ester (CE), we found an increased saturated + monounsaturated/polyunsaturated fatty acid ratio in fasting plasma of low fat fed Cav-1(-/-) mice with increased proportions of CE16:1, CE18:1, CE20:3, and decreased proportions of CE18:2 and CE22:6. Under high fat diet HDL-CE, free cholesterol and pre-beta-HDL were increased accompanied by a shift from slow to fast migrating alpha-HDL and expansion of apoE containing HDL. Our results demonstrate a significant role of Cav-1 in HDL-cholesterol metabolism and may reflect a variety of Cav-1 functions including modulation of ACAT activity and SR-BI function. (c) 2008 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; NULL MICE; CHOLESTEROL ACYLTRANSFERASE-2; ENDOPLASMIC-RETICULUM; DENSITY LIPOPROTEINS; SHOW EVIDENCE; ESI-MS/MS; CLASS-B; MEMBRANE; caveolin-1; caveolae; lipid metabolism; HDL-cholesterol; cholesterol ester |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 10:32 |
| Last Modified: | 05 Nov 2020 10:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31169 |
Actions (login required)
 |
View Item |
