Spitzner, Melanie and Martins, Joana Raquel and Soria, Rene Barro and Ousingsawat, Jiraporn and Scheidt, Kerstin and Schreiber, Rainer and Kunzelmann, Karl (2008) Eag1 and bestrophin 1 are up-regulated in fast-growing colonic cancer cells. JOURNAL OF BIOLOGICAL CHEMISTRY, 283 (12). pp. 7421-7428. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
Ion channels like voltage-gated ether-a-go-go ( Eag1) K+ channels or Ca2+-activated Cl- channels have been shown to support cell proliferation. Bestrophin 1 ( Best1) has been proposed to form Ca2+-activated Cl- channels in epithelial cells. Here we show that original T-84 colonic carcinoma cells grow slowly ( T-84-slow) and express low amounts of Eag1 and Best1, whereas spontaneously transformed T-84 cells grow fast ( T-84-fast) and express high levels of both proteins. Both Eag1 and Best1 currents are up-regulated in T-84-fast cells. Eag1 currents were cell cycle-dependent with up-regulation during G1/ S transition. T-84-slow, but not T-84-fast, cells formed tight monolayers when grown on permeable supports. RNA interference inhibition of Eag1 and Best1 reduced proliferation of T-84-fast cells, whereas overexpression of Best1 turned T-84-slow into fast-growing cells. Eag1 and Best1 improve intracellular Ca2+ signaling and cell volume regulation. These results establish a novel role for bestrophins in cell proliferation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GATED POTASSIUM CHANNELS; CHLORIDE CHANNELS; ION CHANNELS; K+ CHANNELS; ELECTROLYTE TRANSPORT; EPITHELIAL-CELLS; PROLIFERATION; CALCIUM; FAMILY; IDENTIFICATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 10:40 |
| Last Modified: | 05 Nov 2020 10:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31172 |
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