Merkl, Rainer and Zwick, Matthias (2008) H2r: Identification of evolutionary important residues by means of an entropy based analysis of multiple sequence alignments. BMC BIOINFORMATICS, 9: 151. ISSN 1471-2105,
Full text not available from this repository. (Request a copy)Abstract
Background: A multiple sequence alignment (MSA) generated for a protein can be used to characterise residues by means of a statistical analysis of single columns. In addition to the examination of individual positions, the investigation of co-variation of amino acid frequencies offers insights into function and evolution of the protein and residues. Results: We introduce conn(k), a novel parameter for the characterisation of individual residues. For each residue k, conn( k) is the number of most extreme signals of co-evolution. These signals were deduced from a normalised mutual information (MI) value U(k, l) computed for all pairs of residues k, l. We demonstrate that conn(k) is a more robust indicator than an individual MI-value for the prediction of residues most plausibly important for the evolution of a protein. This proposition was inferred by means of statistical methods. It was further confirmed by the analysis of several proteins. A server, which computes conn(k)-values is available at http://www-bioinf.uniregensburg.de. Conclusion: The algorithms H2r, which analyses MSAs and computes conn(k)-values, characterises a specific class of residues. In contrast to strictly conserved ones, these residues possess some flexibility in the composition of side chains. However, their allocation is sensibly balanced with several other positions, as indicated by conn( k).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FUNCTIONALLY IMPORTANT RESIDUES; TRYPTOPHAN SYNTHASE; CORRELATED MUTATIONS; PROTEIN FAMILIES; ALLOSTERIC COMMUNICATION; SALMONELLA-TYPHIMURIUM; CATALYTIC MECHANISM; CRYSTAL-STRUCTURE; SH3 DOMAIN; T-COFFEE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 11:10 |
| Last Modified: | 05 Nov 2020 11:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31174 |
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