Banati, Ferenc and Koroknai, Anita and Salamon, Daniel and Takacs, Maria and Minarovits-Kormuta, Susanna and Wolf, Hans and Niller, Hans Helmut and Minarovits, Janos (2008) CpG-methylation silences the activity of the RNA polymerase III transcribed EBER-1 promoter of Epstein-Barr virus. FEBS LETTERS, 582 (5). pp. 705-709. ISSN 1873-3468,
Full text not available from this repository. (Request a copy)Abstract
CpG-methylation blocks the activity of RNA polymerase 11 transcribed promoters in most cases. In contrast, the role of DNA methylation in the regulation of RNA polymerase III transcribed promoters is less clarified. There are two untranslated viral RNAs (EBER-1 and EBER-2) in most malignant cells carrying latent Epstein-Barr virus (EBV) genomes. We found that in vitro methylation blocked binding of the cellular proteins c-Myc and ATF to the 5'-region of the EBER-1 gene, and silenced the expression of the EBER-1 and EBER-2 genes, transcribed by RNA polymerase 111, in transfected cells. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SEQUENCE-SPECIFIC METHYLATION; LYMPHOMA-DERIVED CELLS; DNA METHYLATION; EXPRESSION; PROTEIN; INHIBITION; ELEMENTS; ENHANCER; REGIONS; BINDING; Epstein-Barr virus; EBER-1; in vitro DNA methylation; c-Myc; ATF; methylation-sensitive protein binding |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2020 11:30 |
| Last Modified: | 05 Nov 2020 11:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31187 |
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