Muesch, Werner and Wege, Anja K. and Maennel, Daniela N. and Hehlgans, Thomas (2008) Generation and characterization of alpha-chymase-Cre transgenic mice. GENESIS, 46 (3). pp. 163-166. ISSN 1526-954X,
Full text not available from this repository. (Request a copy)Abstract
The Cre-loxP technology allows the introduction of somatic gene alterations in a tissue and/or cell type specific manner. The development of transgenes that target Cre expression to specific cell types is a critical component in this system. Here, we describe the generation and characterization of transgenic mouse lines expressing Cre recombinase under the control of the baboon alpha-chymase promoter, designated Chm:Cre, in order to direct Cre expression specifically to mouse mast cells. Chm:Cre expression was detected in mast cells in lung and colon tissue. Cre-mediated recombination in these mice identified a population of mature tissue resident mast cells using ROSA26R reporter mice. No Cre-expression and Cre-mediated recombination was induced in in vitro generated bone marrow derived mast cells or mast cells isolated from the peritoneal cavity indicating that Cre-expression under the control of the alpha-chymase promoter is solely activated in tissue resident mast cells. These Chm:Cre transgenic mice represent a useful tool to specifically inactivate genes of interest in mast cells of these tissues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAST-CELLS; EXPRESSION; PROMOTER; mast cell; Cre; alpha-chymase |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 11:28 |
| Last Modified: | 09 Nov 2020 11:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31256 |
Actions (login required)
 |
View Item |
