Roesch, Alexander and Mueller, Andrea M. and Stempfl, Thomas and Moehle, Christoph and Landthaler, Michael and Vogt, Thomas (2008) RBP2-H1/JARID1B is a transcriptional regulator with a tumor suppressive potential in melanoma cells. INTERNATIONAL JOURNAL OF CANCER, 122 (5). pp. 1047-1057. ISSN 0020-7136,
Full text not available from this repository. (Request a copy)Abstract
The RBP2-H1/JARID1B nuclear protein belongs to the ARID family of DNA-binding proteins and is a potential tumor suppressor that is lost during melanoma development. As we have recently shown, one physiological function of RBP2-H1/JARID1B is to exert cell cycle control via maintenance of active retinoblastoma protein. We now add new evidence that RBP2-H1/JARID1B can also directly regulate gene transcription in a reporter assay system, either alone or as part of a multimolecular complex together with the developmental transcription factors FOXG1b and PAX9. In melanoma cells, chromatin immunoprecipitation combined with promoter chip analysis (ChIP-on-chip) suggests a direct binding of re-expressed RBP2-H1/JARID1B to a multitude of human regulatory chromosomal elements (promoters, enhancers and introns). Among those, a set of 23 genes, including the melanoma relevant genes CDK6 and JAG-1 could be confirmed by cDNA microarray analyses to be differentially expressed after RBP2-H1/JARID1B re-expression. In contrast, in nonmelanoma HEK 293 cells, RBP2-H1/JARID1B overexpression only evokes a minor transcriptional response in cDNA microarray analyses. Because the transcriptional regulation in melanoma cells is accompanied by an inhibition of proliferation, an increase in caspase activity and a partial cell cycle arrest in G1/0, our data support an anti-tumorigenic role of RBP2-H1/JARID1B in melanocytic cells. (c) 2007 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BREAST-CANCER; GENE-EXPRESSION; RETINOBLASTOMA PROTEIN; OLIGONUCLEOTIDE ARRAYS; MALIGNANT-MELANOMAS; CUTANEOUS MELANOMA; BINDING-PROTEINS; FAMILY; APOPTOSIS; PLU-1; melanoma; transcription; gene expression; retinoblastoma protein binding protein; apoptosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 11:58 |
| Last Modified: | 09 Nov 2020 11:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31263 |
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