Hoefelschweiger, Bianca K. and Wolfbeis, Otto S. (2008) Probing DNA hybridization in homogeneous solution and at interfaces via measurement of the intrinsic fluorescence decay time of a single label. JOURNAL OF FLUORESCENCE, 18 (2). pp. 413-421. ISSN 1053-0509,
Full text not available from this repository. (Request a copy)Abstract
The hybridization of DNA oligomers including molecular beacons can be detected by measurement of either the decay time or the intensity of a single fluorescent label attached to the end of the respective oligonucleotide. The method works both in solution and solid phase and can distinguish between fully complementary and mismatch sequences as demonstrated for a 15-mer oligonucleotide and a 25-mer molecular beacon. The fluorescence lifetime method is advantageous in (a) requiring a single label (and therefore a single labeling step) only; and (b), being based on measurement of a self-referenced magnitude that is hardly affected by parameters such as fluctuations in light intensity that make measurement of intensity more prone to interferences.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESONANCE ENERGY-TRANSFER; RESOLVED FLUORESCENCE; FLUOROMETRIC ASSAY; MOLECULAR BEACONS; PROBES; DYES; PCR; OLIGONUCLEOTIDES; ELECTROPHORESIS; TECHNOLOGY; DNA assay; fluorescence lifetime; fluorescent label; molecular beacon; affinity assay |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 05:25 |
| Last Modified: | 09 Nov 2020 05:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31276 |
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