Stover, Cordula M. and Luckett, Jeni C. and Echtenacher, Bernd and Dupont, Aline and Figgitt, Sue E. and Brown, Jane and Maennel, Daniela N. and Schwaeble, Wilhelm J. (2008) Properdin plays a protective role in polymicrobial septic peritonitis. JOURNAL OF IMMUNOLOGY, 180 (5). pp. 3313-3318. ISSN 0022-1767, 1550-6606
Full text not available from this repository. (Request a copy)Abstract
Properdin is a positive regulator of complement activation so far known to be instrumental in the survival of infections with certain serotypes of Neisseria meningitidis. We have generated a fully backcrossed properdin-deficient mouse line by conventional gene-specific targeting. In vitro, properdin-deficient serum is impaired in alternative pathway-dependent generation of complement fragment C3b when activated by Escherichia coli DH5 alpha. Properdin-deficient mice and wild-type littermates compare in their levels of C3 and IgM. In an in vivo model of polymicrobial septic peritonitis induced by sublethal cecal ligation and puncture, properdin-deficient mice appear immunocompromised, because they are significantly impaired in their survival compared with wild-type littermates. We further show that properdin localizes to mast cells and that properdin has the ability to directly associate with E. coli DH5 alpha. We conclude that properdin plays a significant role in the outcome of polymicrobial sepsis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLEMENT ACTIVATION; ALTERNATIVE PATHWAY; POSITIVE REGULATOR; SEPSIS; MICE; IDENTIFICATION; DEFICIENCY; CONVERTASE; IMMUNITY; PUNCTURE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 05:34 |
| Last Modified: | 09 Nov 2020 05:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31280 |
Actions (login required)
 |
View Item |
