Gupta, Shipra and Fuchs, Barbara and Schulz-Maronde, Sandra and Heitland, Aleksandra and Escher, Sylvia E. and Mack, Matthias and Tillmann, Hanns-Christian and Braun, Armin and Forssmann, Wolf-Georg and Elsner, Joern and Forssmann, Ulf (2008) Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation. JOURNAL OF LEUKOCYTE BIOLOGY, 83 (3). pp. 765-773. ISSN 0741-5400, 1938-3673
Full text not available from this repository. (Request a copy)Abstract
Modulation of leukocyte recruitment through intervention with chemokine receptors is an attractive, therapeutic strategy. Recently, we have shown that n-Nonanoyl (NNY)-CCL14 internalizes and desensitizes human (h)CCR3, resulting in the inactivation of eosinophils. In this study, we investigated the interaction of NNY-CCL14 with CCR1 and CCR5 and the relevance of these NNY-CCL14 receptors on its in vivo effects in allergic airway inflammation. NNY-CCL14 has inactivating properties on CCR1(+) and CCR5(+) cell lines and primary leukocytes. It desensitizes hCCR1- and hCCR5-mediated calcium release and internalizes these receptors from the cellular surface. Treatment of OVA-sensitized BALB/c mice with NNY-CCL14 resulted in reduced pulmonary inflammation. Above all, it is demonstrated that systemic treatment with NNY-CCL14 down-modulates CCR5 from the surface of lymphocytes in vivo. Although NNY-CCL14 acts on murine lymphocytes and internalizes CCR5, it does not internalize CCR3 on mouse eosinophils, showing species selectivity regarding this particular receptor. Therefore, the inhibitory effects of NNY-CCL14 in murine models of allergic airway inflammation can be assigned to its interaction with CCR5. The presented results substantiate the relevance of CCR5 as a target for allergic airway inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN EOSINOPHILS; T-CELLS; ASTHMA; RECEPTORS; HYPERRESPONSIVENESS; LUNG; EXPRESSION; DISEASE; PREVENTS; RANTES; allergy; chemokines; human; lymphocytes; mouse |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 05:38 |
| Last Modified: | 09 Nov 2020 05:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31281 |
Actions (login required)
 |
View Item |
