Bollmann, Julia and Ortmann, Olaf and Treeck, Oliver (2008) Expression of differentiation-associated gene icb-1 is estrogen-responsive in ovarian and breast cancer cell lines. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 109 (1-2). pp. 16-21. ISSN 0960-0760,
Full text not available from this repository. (Request a copy)Abstract
icb-1 (Clorf38) is a human gene initially described by our group to be upregulated during in vitro differentiation processes of endometrial adenocarcinoma and leukemia cells triggered by different stimuli. We now report presence of a putative imperfect estrogen response element (ERE) in the promoter of icb-1 gene. Given that estrogens are known to regulate cellular differentiation processes of hormone-dependent tissues, we studied whether expression of icb-1 would be regulated by 17-beta (beta) estradiol in breast and ovarian cancer cells. As examined by means of real time PCR, treatment with 17-beta estradiol for at least 24 h resulted in a significant increase of icb-1 transcript levels in ER alpha-positive MCF-7 breast cancer and OVCAR-3 ovarian cancer cells, but not in ER alpha-negative SK-BR-3 and SK-OV-3 cells. Upregulation of icb-1 transcript levels was also observed after treatment with specific ER alpha-agonist PPT and was inhibited by co-treatment with pure antiestrogen ICI 182,780 in MCF-7 and OVCAR-3 ovarian cancer cells. Treatment with cycloheximide totally inhibited estrogen effects suggesting that activation of icb-1 gene expression is no ERE-dependent early response but a secondary event requiring protein synthesis. The results of this study demonstrate that transcript levels of differentiation-associated gene icb-1 are estrogen-responsive in breast and ovarian cancer cells in an ER alpha-dependent manner. Whether icb-1 is a mediator of estrogen-triggered cellular differentiation processes has to be determined in further studies. (C) 2007 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROGESTERONE-RECEPTOR; ELEMENT; PROLIFERATION; ACTIVATION; SEQUENCE; ALPHA; BETA; MICE; PCR; C1orf38 gene; 17-beta estradiol; breast cancer cell; ovarian cancer cell |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 05:48 |
| Last Modified: | 09 Nov 2020 05:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31288 |
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