Schmid, Andreas and Bala, Margarita and Leszczak, Stephanie and Ober, Irene and Buechler, Christa and Karrasch, Thomas (2016) Pro-inflammatory chemokines CCL2, chemerin, IP-10 and RANTES in human serum during an oral lipid tolerance test. CYTOKINE, 80. pp. 56-63. ISSN 1043-4666, 1096-0023
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Background: There is a strong coincidence of obesity and a chronic state of modest inflammation. Secretion of pro-inflammatory cytokines from adipocytes and immune cells represents a key mechanism in this process and is affected by fatty acids. Material and methods: A study cohort of 100 overnight fasted healthy volunteers underwent an oral lipid tolerance test (OLTT) by ingestion of 160 ml of a protein- and sugar-free lipid emulsion of defined composition. Venal blood was drawn at 0 h (fasting) and at 2, 4, and 6 h after lipid ingestion. Subjects were characterized by anthropometric and standard laboratory parameters. Serum concentrations. of CCL2, IP-10, chemerin, and RANTES were measured by enzyme-linked immunosorbent assay (ELISA). Murine 3T3-L1 adipocytes were stimulated with free fatty acids (FA) and with sex steroids and concentrations of CCL2 and chemerin in cell culture supernatants were measured by ELISA. Results: A significant reduction of circulating CCL2, IP-10, and chemerin concentrations was observed as a consequence of triglyceride ingestion whereas RANTES levels were increased. CCL2 serum concentrations were positively correlated with resistin and visfatin levels and with LDL/HDL ratio and negatively with adiponectin. There were significant differences in chemerin and RANTES serum concentrations in female and male subjects. CCL2 secretion from 3T3-L1 adipocytes was inhibited by treatment with linoleic (LA) and oleic acid (OA) whereas chemerin secretion was induced. Chemerin release from 3T3-L1 adipocytes was inhibited by testosterone. Conclusions: Oral lipid loading is linked to reduced circulating pro-inflammatory chemokines CCL2, IP-10, and chemerin and to increased RANTES levels, suggesting that dietary lipids affect immune function. (C) 2016 Published by Elsevier Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY-ACID STIMULATION; SHORT-TERM REGULATION; METABOLIC SYNDROME; ADIPOSE-TISSUE; INSULIN-RESISTANCE; IN-VITRO; OBESITY; GLUCOSE; MARKERS; DIET; Inflammation; Chemokine; CCL2; Chemerin; IP-10; RANTES; Oral lipid tolerance test; Hypertriglyceridemia |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2019 14:32 |
| Last Modified: | 25 Mar 2019 09:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3129 |
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