Spitalny, Patrizia and Thomm, Michael (2008) A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea. MOLECULAR MICROBIOLOGY, 67 (5). pp. 958-970. ISSN 0950-382X,
Full text not available from this repository. (Request a copy)Abstract
An archaeal histone gene from the hyperthermophile Pyrococcus furiosus containing four consecutive putative oligo-dT terminator sequences was used as a model system to investigate termination signals and the mechanism of termination in vitro. The archaeal RNA polymerase terminated with high efficiency at the first terminator at 90 degrees C when it contained five to six T residues, at 80 degrees C readthrough was significantly increased. A putative hairpin structure upstream of the first terminator had no effect on termination efficiency. Template competition experiments starting with RNA polymerase molecules engaged in ternary complexes revealed recycling of RNA polymerase from the terminator to the promoter of the same template. This facilitated reinitiation was dependent upon the presence of a terminator sequence suggesting that pausing at the terminator is required for recycling as in the RNA polymerase III system. Replacement of the sequences immediately downstream of the oligo-dT terminator by an AT-rich segment improved termination efficiency. Both AT-rich and GC-rich downstream sequences seemed to impair the facilitated reinitiation pathway. Our data suggest that recycling is dependent on a subtle interplay of pausing of RNA polymerase at the terminator and RNA polymerase translocation beyond the oligo-dT termination signal that is dramatically affected by downstream sequences.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TERMINATION SIGNAL RECOGNITION; CELL-FREE TRANSCRIPTION; ESCHERICHIA-COLI RNA; PYROCOCCUS-FURIOSUS; TFIIE-ALPHA; DNA; INITIATION; SEQUENCES; ELONGATION; COMPLEXES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 07:46 |
| Last Modified: | 09 Nov 2020 07:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31304 |
Actions (login required)
 |
View Item |
