Hausmann, M. and Paul, G. and Menzel, K. and Brunner-Ploss, R. and Falk, W. and Schoemerich, J. and Herfarth, H. and Rogler, G. (2008) NAT1 genotypes do not predict response to mesalamine in patients with ulcerative colitis. ZEITSCHRIFT FUR GASTROENTEROLOGIE, 46 (3). pp. 259-265. ISSN 0044-2771,
Full text not available from this repository. (Request a copy)Abstract
Background: 5-Aminosalicylic acid (5-ASA) is metabolised in colonic mucosa by N-acetyltransferase 1 (NAT1). Common genetic polymorphisms in this enzyme result in rapid or slow acetylation. 5-ASA treatment causes side effects in up to 10% of patients with ulcerative colitis (UC). We therefore determined genetic variations of NAT1 in patients with UC and looked for a possible association with the clinical response to 5-ASA. Methods: DNA was obtained from 78 patients with UC. 77% of the patients were in remission during 5-ASA treatment, whereas 23% suffered from active disease. NAT1 genotyping was performed for 23 known alleles using RFLP and sequence analysis. Clinical response to 5-ASA was determined by medical record review and associated with NAT1 genotypes. Results: Utilising PCR we amplified a 570-bp coding region of the human NAT1 gene in addition to 240 bp in the 3'-untranslated region (UTR). 4 NAT1 alleles previously known as NAT1 *3, *4, *10 and *11 were recovered. 31% of the patients were heterozygous and 4% homozygous for the NAT1*10 allele. 6% were heterozygous for the NAT1*3 allele. 6% were heterozygous for the NAT1*11 allele. No association was found between NAT1 genotype and clinical response as well as side effects to 5-ASA in patients with UC. Conclusions: NAT1 genotypes do not predict response or side effects to mesalamine in patients with UC. Variations caused by non-genomic effects may be associated with the clinical response to 5-ASA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARYLAMINE N-ACETYLTRANSFERASE; INFLAMMATORY-BOWEL-DISEASE; 5-AMINOSALICYLIC ACID; METABOLIC-ACTIVATION; SMALL-INTESTINE; TYPE-1 NAT1; HUMAN LIVER; ACETYLATION; POLYMORPHISM; RELEASE; ulcerative colitis; Crohn's disease; intestinal complications |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 09:51 |
| Last Modified: | 09 Nov 2020 09:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31358 |
Actions (login required)
 |
View Item |
