Straub, Rainer H. and Wolff, Christine and Fassold, Alexander and Hofbauer, Rafael and Chover-Gonzalez, Antonio and Richards, Louise J. and Jessop, David S. (2008) Antiinflammatory role of endomorphins in osteoarthritis, rheumatoid arthritis, and adjuvant-induced polyarthritis. ARTHRITIS AND RHEUMATISM, 58 (2). pp. 456-466. ISSN 0004-3591,
Full text not available from this repository. (Request a copy)Abstract
Objective. Pain sensitization and the related secretion of neuropeptides from sensory nerve terminals are proinflammatory in osteoarthritis (OA), rheumatoid arthritis (RA), and adjuvant-induced polyarthritis. In contrast, endogenous opioids such as the recently discovered endomorphins (EMs) are antiinflammatory. However, the role of endogenous EMs such as EM-I and EM-2 has never been investigated in OA and RA. Methods. We established a highly sensitive radioimmunoassay to detect EM-1 and EM-2. In patients with RA and patients with OA, immunohistochemistry for EM-1 and EM-2 was performed, and double-staining was used to identify EM-positive cells. The effects of EM-1 and EM-2 on the secretion of interleukin-6 (IL-6) and IL-8 from human synovial tissue were studied by tissue superfusion, and the therapeutic effects of EM-1 were tested in a rat model of adjuvant-induced polyarthritis. Results. EM-positive cells were located in the sublining area and vessel walls but were particularly evident in the highly inflamed lining area. Human macrophages, T cells, and fibroblasts stained positive for EMs. The synovial density of EM-positive cells was higher in patients with OA than in those with RA. EM-1 inhibited synovial secretion of IL-6 in patients with RA and secretion of IL-8 in patients with RA and those with OA (maximum 10(-10)M). EM-2 inhibited IL-8 secretion only from RA tissue (maximum 10(-10)M). In rats with adjuvant-induced polyarthritis, thymus, spleen, and synovial tissue contained significantly more EM-1 than was observed in controls. Rats with adjuvant-induced polyarthritis benefited from EM-1 treatment. Conclusion. EM-1 had antiinflammatory effects in patients with OA or RA and in a model of adjuvant-induced polyarthritis. Local enhancement of EM-1 might be an interesting therapeutic option in different forms of arthritis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRODYNORPHIN GENE-EXPRESSION; COLLAGEN-INDUCED ARTHRITIS; SYMPATHETIC-NERVE FIBERS; MU-OPIOID RECEPTORS; SPINAL-CORD; BETA-ENDORPHIN; SUBSTANCE-P; MET-ENKEPHALIN; ADMINISTERED ENDOMORPHIN-1; INTERLEUKIN-6 SECRETION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Nov 2020 06:37 |
| Last Modified: | 10 Nov 2020 06:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31392 |
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