Hahtola, Sonja and Burghart, Elke and Puputti, Marjut and Karenko, Leena and Abdel-Rahman, Wael M. and Vakeva, Liisa and Jeskanen, Leila and Virolainen, Susanna and Karvonen, Jaakko and Salmenkivi, Kaisa and Kinnula, Vuokko and Joensuu, Heikki and Peltorndki, Paivi and Klein, Christoph A. and Ranki, Annamari (2008) Cutaneous T-cell lymphoma-associated lung cancers show chromosomal aberrations differing from primary lung cancer. GENES CHROMOSOMES & CANCER, 47 (2). pp. 107-117. ISSN 1045-2257,
Full text not available from this repository. (Request a copy)Abstract
Cutaneous T-cell lymphoma (CTCL) patients have an increased risk of certain secondary cancers, the most common of which are lung cancers, especially small cell lung cancer. To reveal the molecular pathogenesis underlying CTCL-associated lung cancer, we analyzed genomic aberrations in CTCL-associated and reference lung cancer samples. DNA derived from microdissected lung cancer cells of five CTCL-associated lung cancers and five reference lung cancers without CTCL association was analyzed by comparative genomic hybridization (CGH). Fluorescent in situ hybridization (FISH), immunohistochemistry (IHC), and loss of heterozygosity (LOH) analysis were performed for selected genes. In CTCL-associated lung cancer, CGH revealed chromosomal aberrations characterizing both lung cancer and CTCL, but also losses of I p, and 19, and gains of 4q and 7, hallmarks of CTCL. LOH for the CTCL-associated NAV3 gene was detected in two of the four informative primary lung cancers. FISH revealed increased copy number of the KIT gene in 3/4 of CTCL-associated lung cancers and 1/5 of primary lung cancers. PDGFRA and VEGFR2 copy numbers were also increased. IHC showed moderate KIT expression when the gene copy number was increased. CTCL-associated lung cancer shows chromosomal aberrations different from primary lung cancer, especially amplifications of 4q, a chromosome arm frequently deleted in the latter tumor type. Copy numbers and expression of selected genes in chromosome 4 differed between CTCL-associated and reference lung cancers. These preliminary observations warrant further prospective studies to identify the common underlying factors between CTCL and CTCL-associated lung cancer. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. (c) 2007 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-RECEPTOR; COMPARATIVE GENOMIC HYBRIDIZATION; SEZARY-SYNDROME; TYROSINE-KINASES; NERVOUS-SYSTEM; KIT EXPRESSION; PDGFR-ALPHA; AMPLIFICATION; CARCINOMA; TUMORS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2020 12:51 |
| Last Modified: | 09 Nov 2020 12:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31415 |
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