Beutner, Andrea and Cunha, Rafael Rodrigues and Richter, Eduardo Mathias and Matysik, Frank-Michael (2016) Combining (CD)-D-4 and MS as a dual detection approach for capillary electrophoresis. ELECTROPHORESIS, 37 (7-8). pp. 931-935. ISSN 0173-0835, 1522-2683
Full text not available from this repository. (Request a copy)Abstract
The hyphenation of two detectors in combination with separation techniques is a powerful tool to enhance the analytical information. In this work, we present for the first time the coupling of two important detectors for capillary electrophoresis (CE), namely capacitively coupled contactless conductivity detection ((CD)-D-4) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS). The elaborated experimental protocol took into account the requirements of separation aspects and the compatibility with both detectors. ESI-TOFMS requires background electrolytes (BGE) containing only volatile components such as ammonium acetate or formate. These, however, exhibit a rather high conductivity, which is disadvantageous for (CD)-D-4. Thus, the selection of the BGE in an appropriate concentration was undertaken for the determination of various phenolic compounds serving as a model system. The chosen BGE was a 10 mu M ammonium acetate/ammonia buffer with a pH of 9. This BGE was a compromise concerning the detection performance of both detectors. The LODs for m-cresol, m-and p-nitrophenol, and 2,4-dinitrophenol were 3.1 mu M ((CD)-D-4), 0.8 mu M (MS), 0.8 mu M (MS), and 1.5 mu M (MS), respectively. Moreover, the overall separation efficiency was excellent illustrating that detector-induced band broadening can be neglected in the CE-(CD)-D-4/MS system. The analytical characteristics for the determination of phenolic compounds show the suitability of this dual detection approach and demonstrate the complementary use of (CD)-D-4 and MS detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONTACTLESS CONDUCTIVITY DETECTION; ELECTROCHEMICAL DETECTION; MICROCHIP ELECTROPHORESIS; ZONE-ELECTROPHORESIS; MASS-SPECTROMETRY; BATCH INJECTION; SEPARATION; FLUORESCENCE; DICLOFENAC; CE; Capacitively coupled contactless conductivity detection; Capillary electrophoresis; Dual detection; Mass spectrometry; Phenols |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2019 06:56 |
| Last Modified: | 22 Mar 2019 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3148 |
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