Boecker, Daniel and Zybin, Alexander and Niemax, Kay and Grunwald, Christian and Mirsky, Vladimir M. (2008) Noise reduction by multiple referencing in surface plasmon resonance imaging. REVIEW OF SCIENTIFIC INSTRUMENTS, 79 (2): 023110. ISSN 0034-6748, 1089-7623
Full text not available from this repository. (Request a copy)Abstract
The analytical performance of surface plasmon resonance imaging with charge coupled device detection can be improved significantly by splitting a macroscopic sensing surface into multiple microscopic neighboring sensing and referencing subareas. It is shown that such a multiple referencing reduces intensity fluctuations across the total sensing area and, therefore, improves the signal/noise (S/N) ratio proportional to the splitting factor. The approach is demonstrated by detection of biotin binding to a monolayer of streptavidin. An effective variation of the reflected intensity of about 10(-4), which corresponds to the refraction index variation of 3 x 10(-6), was detected with a S/N ratio about 10 without any temperature stabilization of the sensing area. (C) 2008 American Institute of Physics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROSCOPY; ARRAYS; DNA; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Nov 2020 10:05 |
| Last Modified: | 10 Nov 2020 10:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31484 |
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