Alles, Julia and Menegatti, Jennifer and Motsch, Natalie and Hart, Martin and Eichner, Norbert and Reinhardt, Richard and Meister, Gunter and Graesser, Friedrich A. (2016) miRNA expression profiling of Epstein-Barr virus-associated NKTL cell lines by Illumina deep sequencing. FEBS OPEN BIO, 6 (4). pp. 251-263. ISSN 2211-5463,
Full text not available from this repository. (Request a copy)Abstract
The aim of this work was to establish the microRNA profile of SNK6 and SNT16, two Epstein-Barr virus (EBV)-infected cell lines derived from nasal NK/T-cell lymphoma (NKTL). The oncogenic EBV is strongly associated with the pathogenesis of nasal and extranodal NK/T-cell lymphoma and expresses 44 mature microRNAs and two noncoding EBV-encoded RNAs (EBERs). miRNAs are 19-25nt noncoding RNAs that affect host and viral gene expression post-transcriptionally. Deregulated miRNA patterns are frequently linked to a variety of human cancers including lymphomas. miRNA profiling of the two NK/T cell lines vs. primary cells revealed 10 and 4 up-regulated and 10 and 12 down-regulated miRNAs in SNK6 and SNT16 cells respectively. The results were validated by qRT-PCR for selected miRNAs. Target gene analyses confirmed cullin 5 (CUL5) and sphingosin-1-phosphate receptor 1 (S1PR1) as targets for the down-regulated hsa-miR-148a and viral ebv-miR-BART16 respectively. As recently demonstrated for the regulation of IL1-alpha by miR-142-3p, coexpression of the EBERs selectively exerted corepression of S1PR1 by BART16 but not of CUL5 by miR-148a, indicating selective corepression by the EBERs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTORS; 2 SMALL RNAS; T-CELL; ARGONAUTE PROTEINS; MICRORNA TARGETS; DOWN-REGULATION; GW182 PROTEINS; NASAL; LYMPHOMA; SPHINGOSINE-1-PHOSPHATE; CUL5; ebv-miR-BART16; Epstein-Barr virus; hsa-miR-148a; microRNA; S1PR1 |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 08:52 |
| Last Modified: | 03 Apr 2019 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3150 |
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