Hafner, Christian and Stempfl, Thomas and Baeumler, Wolfgang and Hohenleutner, Ulrich and Landthaler, Michael and Vogt, Thomas (2008) Gene expression profiling of melanocytes following Q-switched Ruby Laser irradiation. DERMATOLOGY, 216 (1). pp. 6-13. ISSN 1018-8665,
Full text not available from this repository. (Request a copy)Abstract
Background: The Q- switched Ruby laser (QSRL) is used for the treatment of pigmented lesions. The influence of QSRL treatment on gene expression of nontransformed primary melanocytes has not been addressed in vitro. Objective: We investigated the gene expression profile of melanocytes following QSRL irradiation. Methods: Primary melanocytes were irradiated with the QSRL (694 nm). Early and late transcriptional effects were analyzed using the Affymetrix gene array platform. Results: Laser irradiation of melanocytes had minor effects on mRNA expression. We found only 31 out of 14,500 genes which were at least twofold up- or downregulated. The differential expression of heme oxygenase 1 and galanin in QSRL- treated melanocytes was additionally confirmed by real-time RTPCR. Analysis of a selection of 36 genes which are known to be associated with malignant melanoma development and progression revealed no significantly aberrant expression in the QSRL- treated melanocytes. Conclusion: Our study shows that QSRL treatment of primary melanocytes in vitro does not cause major alterations of global gene expression and particularly of genes associated with malignant melanoma. However, since QSRL treatment may have different effects on gene expression of melanocytic cells in vivo, further studies are required to evaluate QSRL treatment of (nevo-) melanocytic lesions. Copyright (C) 2008 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELECTIVE PHOTOTHERMOLYSIS; MALIGNANT-MELANOMA; PIGMENTED LESIONS; HEME OXYGENASE-1; HUMAN SKIN; IN-VITRO; ND-YAG; NM; BENIGN; CELLS; Q-switched Ruby Laser; melanocyte; gene expression profile; melanoma |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Nov 2020 12:04 |
| Last Modified: | 11 Nov 2020 12:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31616 |
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