Drechsler, Maik D. and Obermeier, Ingrid and Doring, Yvonne and Lackner, Karl J. and Modrow, Susanne and Landenberg, Philipp von (2008) Generation of multifunctional murine monoclonal antibodies specifically directed to the VP1unique region protein of human parvovirus B19. IMMUNOBIOLOGY, 213 (6). pp. 511-517. ISSN 0171-2985,
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Little is known about the VP1 unique region (VP1u), a part of one major capsid protein of human parvovirus B19 (B19), conccerning its involvement in viral replication and infection cycle. Showing a phospholipase A(2) (PLA(2))-like activity, which is discussed to be necessary for viral release from host cell, its precise function remains unclear. The purpose of this study was to generate multifunctional monoclonal antibodies (mabs) for different applications that may be useful in investigating VP1u's relevance. To establish antiVP1u antibodies, spleen cells from Balb/c mice immunized with purified recombinant viral protein were used for generating antibody-producing hybridoma cell line. Usability of the antibodies was tested in enzyme-linked immunosorbent assay (ELISA), Western-blot analysis, immunofluorescence and an inhibition assay of enzymatic activity of PLA(2). Three hybridoma cell lines secreting mab's specifically directed against the VP1u protein of B19 could be generated and functioned in every screening method used in this study. These antibodies are helpful tools for investigations in B19 research and diagnosis. Furthermore, the antibodies could help in gaining a deeper understanding of VP1u's role in viral replication and infection especially in the importance of its constitutive PLA(2)-like activity. (C) 2008 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MINOR CAPSID PROTEIN; UNIQUE REGION; B19-ASSOCIATED MYOCARDITIS; PHOSPHOLIPASE A(2); VP1-UNIQUE REGION; RHEUMATIC-DISEASE; INFECTION; IDENTIFICATION; EXPRESSION; VP1; human parvovirus B19; monoclonal antibody; VP1unique region protein; VP1u; phospholipase A(2)-like activity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Nov 2020 12:20 |
| Last Modified: | 13 Nov 2020 12:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31656 |
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