Micorescu, Michael and Grunberg, Sebastian and Franke, Andreas and Cramer, Patrick and Thomm, Michael and Bartlett, Michael (2008) Archaeal transcription: Function of an alternative transcription factor B from Pyrococcus furiosus. JOURNAL OF BACTERIOLOGY, 190 (1). pp. 157-167. ISSN 0021-9193, 1098-5530
Full text not available from this repository. (Request a copy)Abstract
The genome of the hyperthermophile archaeon Pyrococcus furiosus encodes two transcription factor B (TFB) paralogs, one of which (TFB1) was previously characterized in transcription initiation. The second TFB (TFB2) is unusual in that it lacks recognizable homology to the archaeal TFB/eukaryotic TFIIB B-finger motif. TFB2 functions poorly in promoter-dependent transcription initiation, but photochemical cross-linking experiments indicated that the orientation and occupancy of transcription complexes formed with TFB2 at the strong gdh promoter are similar to the orientation and occupancy of transcription complexes formed with TFB1. Initiation complexes formed by TFB2 display a promoter opening defect that can be bypassed with a preformed transcription bubble, suggesting a mechanism to explain the low TFB2 transcription activity. Domain swaps between TFB1 and TFB2 showed that the low activity of TFB2 is determined mainly by its N terminus. The low activity of TFB2 in promoter opening and transcription can be partially relieved by transcription factor E (TFE). The results indicate that the TFB N-terminal region, containing conserved Zn ribbon and B-finger motifs, is important in promoter opening and that TFE can compensate for defects in the N terminus through enhancement of promoter opening.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TATA-BINDING PROTEIN; RNA-POLYMERASE-II; DNA MICROARRAY ANALYSIS; HIGHLY EXPRESSED GENES; HEAT-SHOCK RESPONSE; PREINITIATION COMPLEX; HYPERTHERMOPHILIC ARCHAEON; INITIATION COMPLEX; STRUCTURAL BASIS; TFIIB HOMOLOG; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Nov 2020 13:05 |
| Last Modified: | 13 Nov 2020 13:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31674 |
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