Lehle, Karla and Schreml, Stephan and Kunz-Schughart, Leoni A. and Rupprecht, Leopold and Birnbaum, Dietrich E. and Schmid, Christof and Preuner, Juergen G. (2008) mTOR inhibitors and calcineurin inhibitors do not affect adhesion molecule expression of human macro- and microvascular endothelial cells. JOURNAL OF VASCULAR RESEARCH, 45 (4). pp. 333-342. ISSN 1018-1172,
Full text not available from this repository. (Request a copy)Abstract
We examined the effect of cyclosporin A, tacrolimus, sirolimus and everolimus on the cell growth, viability, proliferation, expression of cellular adhesion molecules (CAM) and leukocyte (PBMC) binding of human macrovascular (coronary artery, saphenous vein) and microvascular endothelial cells (EC). Tacrolimus did not affect EC integrity, growth or expression of CAM. Exclusively, EC from the coronary arteries showed a reduced cellular growth (about 30%) under cyclosporin A and tacrolimus treatment. In contrast, treatment with mTOR inhibitors reduced EC proliferative activity by about 40%, independently of the EC origin. No induction of apoptosis (caspase-3/7 activity) or cytotoxicity (MTS test) was observed. Long-term treatment with high concentrations of sirolimus and everolimus did not enhance the expression of CAM. Stimulation with tumor necrosis factor significantly increased the expression of CAM, independently of the drugs used. None of the mTOR inhibitors influenced the tumor necrosis factor-induced expression of CAM, whereas adhesion of PBMC increased significantly, as described by other papers. In summary, neither calcineurin inhibitors nor mTOR inhibitors activate human micro- and macrovascular EC. Therefore, the investigated drugs are unlikely to contribute to EC activation during transplant-associated vasculopathy. Copyright (C) 2008 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARDIAC ALLOGRAFT VASCULOPATHY; CYCLOSPORINE-A; IN-VITRO; IMMUNOSUPPRESSIVE REGIMENS; MAMMALIAN TARGET; MITOMYCIN-C; RAPAMYCIN; PROLIFERATION; DYSFUNCTION; ACTIVATION; adhesion molecules; calcineurin inhibitors; mTOR inhibitors; E-selectin; ICAM-1; VCAM-1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Nov 2020 10:22 |
| Last Modified: | 18 Nov 2020 10:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31700 |
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