Denzinger, Stefan and Burger, Maximilian and Hammerschmied, Christine G. and Wieland, Wolf F. and Hartmann, Arndt and Obermann, Ellen C. and Stoehr, Robert (2008) Pax-5 protein expression in bladder cancer: a preliminary study that shows no correlation to grade, stage or clinical outcome. PATHOLOGY, 40 (5). pp. 465-469. ISSN 0031-3025,
Full text not available from this repository. (Request a copy)Abstract
Aims: Pax (paired box) genes comprise a gene family crucial for cell differentiation that encodes a set of transcription factors. Recently, Pax-5 mRNA expression was suggested as a prognostic marker in bladder cancer (BC). However, a functional role of Pax-5 in BC is questionable because the protein expression was not determined in these studies. Therefore, we evaluated Pax-5 protein expression in an unselected, consecutive series of BC. Methods: We immunohistochemically investigated Pax-5 protein expression in 100 archival bladder tumours and 22 normal urothelial samples using tissue microarray (TMA) technology and a monoclonal antibody against Pax-5. Staining intensity and percentage of positively stained cells were determined and correlated to histopathological characteristics of the tumours and clinical follow-up data. Results: All 22 samples of histopathologically normal urothelium were negative for Pax-5 protein expression. Overall, 70 of 100 tumours gave interpretable results. Only seven of 70 (10%) cases showed a positive nuclear Pax-5 staining but without significant correlation to clinicopathological characteristics. Interestingly, we could observe Pax-5 positive lymphocytes located within the tumour or closely adjacent in the underlying stroma in 24 of 70 (34%) cases in our series. Conclusions: Pax-5 protein expression is infrequent in BC. Absence of correlation to clinicopathological characteristics suggests a minor functional role of Pax-5 in BC. Pax-5 positive lymphocytes within reactive infiltrates adjacent to the tumour warrant further studies evaluating biological, immunological and clinical relevance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSITIONAL-CELL CARCINOMA; BREAST-CANCER; GENES; PROGRESSION; TUMORS; INFLAMMATION; ASSOCIATION; PROGNOSIS; SURVIVAL; LEUKEMIA; bladder cancer; tissue microarray; Pax-5; immunohistochemistry |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Urologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Nov 2020 07:52 |
| Last Modified: | 23 Nov 2020 07:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31744 |
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