Morsczeck, Christian and Korenkov, Michael and Nagelschmidt, Manfred and Feher, Domonkos and Schierholz, Joerg Michael (2008) Total RNA-isolation of abdominal hernia of rats for quantitative real-time reverse transcription (RT) PCR assays. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 38 (1). pp. 87-93. ISSN 1082-6068,
Full text not available from this repository. (Request a copy)Abstract
Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA-free total RNA of incisional hernias for the first time. Moreover, real-time RT PCR assays for collagen type I and III and TGF-beta 1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE-EXPRESSION; real-time RT-PCR; hernia; rat; RNA-isolation; surgery; collagen |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Nov 2020 10:26 |
| Last Modified: | 23 Nov 2020 10:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31764 |
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