Ladewig, Julia and Koch, Philipp and Endl, Elmar and Meiners, Banu and Opitz, Thoralf and Couillard-Despres, Sebastien and Aigner, Ludwig and Bruestle, Oliver (2008) Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons. STEM CELLS, 26 (7). pp. 1705-1712. ISSN 1066-5099, 1549-4918
Full text not available from this repository. (Request a copy)Abstract
A major prerequisite for the biomedical application of human embryonic stem cells (hESC) is the derivation of defined and homogeneous somatic cell types. Here we present a human doublecortin (DCX) promoter-based lineage-selection strategy for the generation of purified hESC-derived immature neurons. After transfection of hESC-derived neural precursors with a DCX-enhanced green fluorescent protein construct, fluorescence-activated cell sorting enables the enrichment of immature human neurons at purities of up to 95%. Selected neurons undergo functional maturation and are able to establish synaptic connections. Considering that the applicability of purified hESC-derived neurons would largely benefit from an efficient cryopreservation technique, we set out to devise defined freezing conditions involving caspase inhibition, which yield post-thaw recovery rates of up to 83%. Combined with our lineage-selection procedure this cryopreservation technique enables the generation of human neurons in a ready-to-use format for a large variety of biomedical applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROTUBULE-ASSOCIATED PROTEIN; IN-VITRO DIFFERENTIATION; YOUNG NEURONS; FREEZE-THAW; DOUBLECORTIN; EXPRESSION; BRAIN; TRANSPLANTATION; INTEGRATION; PRECURSORS; human embryonic stem cells; lineage selection; human neurons; cryopreservation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Nov 2020 13:32 |
| Last Modified: | 23 Nov 2020 13:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31780 |
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