Wieneke, Ralph and Klein, Susanne and Geyer, Armin and Loos, Eckhard (2007) Structural and functional characterization of galactooligosaccharides in Nostoc commune: beta-D-galactofuranosyl-(1 -> 6)-[beta-D-galactofuranosyl-(1 -> 6)](2)-beta-D-1,4-anhydrogalactitol and beta-(1 -> 6)-galactofuranosylated homologues. CARBOHYDRATE RESEARCH, 342 (18). pp. 2757-2765. ISSN 0008-6215,
Full text not available from this repository. (Request a copy)Abstract
A new class of galactooligosaccharides has been identified from the terrestrial cyanobacterium Nostoc commune by MS and NMR techniques. These consist Of beta-D-galactofuranosyl-(1--->6)-[beta-D-galactofuranosyl-(1-->6)](n)-beta-D-1,4-anhydrogalactitols with n ranging from 2 to 8, corresponding to compounds designated I through 7. In total these saccharides amounted to similar to 0.35% of the dry thallus of N. commune, while in several other cyanobacteria they were not detected. Possibly they play some role in protection from damage by heat and desiccation as suggested by experiments with heterologous systems. For example, phosphoglucomutase (EC 2.7.5.1) from rabbit muscle was protected against heat inactivation by these oligosaccharides, and alpha-amylase (EC 3.2.1.1) from porcine pancreas by the oligosaccharides 6 and 7. The homologues of lower molecular mass, however, enhanced heat sensitivity of a-amylase. The viability of Escherichia coli was completely abolished by desiccation, whereas in the presence of 4 survival rates were similar to 50% of controls not subjected to desiccation. The newly identified saccharides are compared with known galactofuranose-based oligo- and polysaccharides and possible biological functions of them are discussed. (C) 2007 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-WALL POLYSACCHARIDES; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; INTRACELLULAR TREHALOSE; CHEMICAL-STRUCTURE; PENICILLIUM; IMMUNODOMINANT; STABILITY; PROTEINS; FUSARIUM; oligosaccharides; galactofuranose; D-1,4-anhydrogalactitol; Nostoc commune; enzymes; heat protection; Escherichia coli; desiccation |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Nov 2020 06:21 |
| Last Modified: | 25 Nov 2020 06:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31795 |
Actions (login required)
 |
View Item |
