Grunberg, Sebastian and Bartlett, Michael S. and Naji, Souad and Thomm, Michael (2007) Transcription factor E is a part of transcription elongation complexes. JOURNAL OF BIOLOGICAL CHEMISTRY, 282 (49). pp. 35482-35490. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
A homologue of the N-terminal domain of the alpha subunit of the general eukaryotic transcription factor TFE is encoded in the genomes of all sequenced archaea, but the position of archaeal TFE in transcription complexes has not yet been defined. We show here that TFE binds nonspecifically to single-stranded DNA, and photochemical cross-linking revealed TFE binding to a preformed open transcription bubble. In preinitiation complexes, the N-terminal part of TFE containing a winged helix-turn-helix motif is cross-linked specifically to DNA of the non-template DNA strand at positions -9 and -11. In complexes stalled at +20, TFE cross-linked specifically to positions +9, +11, and +16 of the non-template strand. Analyses of transcription complexes stalled at position +20 revealed a TFE-dependent increase of the resumption efficiency of stalled RNA polymerase and a TFE-induced enhanced permanganate sensitivity of thymine residues in the transcription bubble. These results demonstrate the presence of TFE in early elongation complexes and suggest a role of TFE in stabilization of the transcription bubble during elongation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-II; ARCHAEAL TRANSCRIPTION; PREINITIATION COMPLEX; BASAL TRANSCRIPTION; INITIATION COMPLEX; TFIIE-ALPHA; PROMOTER CLEARANCE; CROSS-LINKING; DNA; REVEALS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Nov 2020 06:56 |
| Last Modified: | 25 Nov 2020 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31807 |
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