Sancilio, Silvia and Gallorini, Marialucia and Cataldi, Amelia and di Giacomo, Viviana (2016) Cytotoxicity and apoptosis induction by e-cigarette fluids in human gingival fibroblasts. CLINICAL ORAL INVESTIGATIONS, 20 (3). pp. 477-483. ISSN 1432-6981, 1436-3771
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Electronic cigarettes (e-cigarettes) are generally acknowledged as a safer alternative to the use of combusted tobacco products. Nevertheless, there are increasing conflicting claims concerning the effect of these novel industrial products on the health of e-cigarettes users. The aim of this work was to investigate the effects of the liquids of e-cigarettes on human gingival fibroblasts (HGFs) and to compare the effects of nicotine-containing fluid to the fluid itself. HGFs were treated with different concentrations (0-5 mg/mL) of fluids of e-cigarettes for different times (0-72 h) and cytotoxicity was analyzed by MTT assay. Fluids were administered also after being vaped (e.g., warmed into the cartomizer). Apoptosis occurrence and Bax expression were evaluated by flow cytometry; ROS production was analyzed by fluorescence optical microscopy. Both nicotine-containing and nicotine-free fluids induced an increased ROS production after 24 h, along with an increased Bax expression, followed by apoptosis occurrence after 48 h of exposure. The cytotoxicity exerted on HGFs by e-cigarettes fluids is not entirely ascribable to nicotine. Since the e-cigarettes are advertised as a safer alternative to traditional ones, especially for the possibility of "smoking" nicotine-free fluids, further studies are necessary to clarify the mechanism involved in the occurrence of cytotoxicity exerted by such compounds. Our results suggest a role for e-cigarette fluids in the pathogenesis of oral diseases, such as periodontitis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VITRO; GENE-EXPRESSION; NICOTINE; SMOKE; VIABILITY; MIGRATION; DISEASE; CANCER; CELLS; LUNG; e-cigarette; Reactive oxygen species; Bax; Apoptosis; Human gingival fibroblast; Cytotoxicity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnärztliche Prothetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 09:27 |
| Last Modified: | 03 Apr 2019 09:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3182 |
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