Grassinger, Jochen and Simon, Michaela and Mueller, Gunnar and Drewel, Diana and Andreesen, Reinhard and Hennemann, Burkhard (2007) Bone morphogenetic protein (BMP)-7 but not BMP-2 and BMP-4 improves maintenance of primitive peripheral blood-derived hematopoietic progenitor cells (HPC) cultured in serum-free medium supplemented with early acting cytokines. CYTOKINE, 40 (3). pp. 165-171. ISSN 1043-4666, 1096-0023
Full text not available from this repository. (Request a copy)Abstract
BMPs regulate the developmental program of hematopoiesis. We demonstrate an increased expression of the BMP receptors la and 11 on cultured CD34(+) cells and examine the impact of BMP-2, -4 and -7 on postnatal HPC cultured with stem cell factor, flt3-ligand and interleukin-3 (SF3). The addition of BMP-2 at 5, 25 and 50 ng/m to serum-free medium with SF3 yielded a 1.4- to 1.2-fold increase of CD34(+) cells after seven days, but no effect on CFC or LTC-IC was observed. BMP-4 at 25 ng/ml induced a 2.9-fold expansion of colony-forming cells (CFC) within I week followed by a decrease to pre-culture values on day 14. The number of long-term culture initiating cells (LTC-IC) decreased by the factor 40 from day 0 to day 14. BMP-7 at 5-50 ng/ml had not effect on the expansion of CD34(+) cells and CFC, but improved at 5 ng/ml the survival of LTC-IC significantly as compared to SF3 alone. In summary, BMP-2, -4 and -7 have no effect on the proliferation of CD34(+) cells and CFC cultured with serum-free medium and SF3. However, BMP-7 but not BMP-2 and BMP-4 prevents the loss of primitive hematopoietic progenitor cells cultured in SFM plus SF3. (c) 2007 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STEM-CELLS; DIFFERENTIATION; EXPANSION; RESPONSES; MEMBERS; GROWTH; hematopoiesis; hematopoietic progenitor cells; bone morphogenetic protein; cell differentiation; cell proliferation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Nov 2020 09:17 |
| Last Modified: | 25 Nov 2020 09:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31830 |
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