Lukas, Karin and Thomas, Ulrich and Gessner, Andre and Wehner, Daniel and Schmid, Thomas and Schmid, Christof and Lehle, Karla (2016) Plasma functionalization of polycarbonaturethane to improve endothelializationEffect of shear stress as a critical factor for biocompatibility control. JOURNAL OF BIOMATERIALS APPLICATIONS, 30 (9). pp. 1417-1428. ISSN 0885-3282, 1530-8022
Full text not available from this repository. (Request a copy)Abstract
Medical devices made of polycarbonaturethane (PCU) combine excellent mechanical properties and little biological degradation, but restricted hemocompatibility. Modifications of PCU might reduce platelet adhesion and promote stable endothelialization. PCU was modified using gas plasma treatment, binding of hydrogels, and coupling of cell-active molecules (modified heparin, anti-thrombin III (ATIII), argatroban, fibronectin, laminin-nonapeptide, peptides with integrin-binding arginine-glycine-aspartic acid (RGD) motif). Biocompatibility was verified with static and dynamic cell culture techniques. Blinded analysis focused on improvement in endothelial cell (EC) adhesion/proliferation, anti-thrombogenicity, reproducible manufacturing process, and shear stress tolerance of ECs. EC adhesion and antithrombogenicity were achieved with 9/35 modifications. Additionally, 6/9 stimulated EC proliferation and 3/6 modification processes were highly reproducible for endothelialization. The latter modifications comprised immobilization of ATIII (A), polyethyleneglycole-diamine-hydrogel (E) and polyethylenimine-hydrogel connected with modified heparin (IH). Under sheer stress, only the IH modification improved EC adhesion within the graft. However, ECs did not arrange in flow direction and cell anchorage was restricted. Despite large variation in surface modification chemistry and improved EC adhesion under static culture conditions, additional introduction of shear stress foiled promising preliminary data. Therefore, biocompatibility testing required not only static tests but also usage of physiological conditions such as shear stress in the case of vascular grafts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLY(ETHYLENE GLYCOL) HYDROGELS; IN-VITRO; ENDOTHELIAL-CELLS; PLATELET-ADHESION; SURFACE MODIFICATION; PROTEIN ADSORPTION; POLYMER SURFACES; VASCULAR GRAFTS; BYPASS GRAFT; GAS PLASMA; Endothelial cell seeding; thrombogenicity; plasma treatment; hydrogel; polyurethane; sheer stress; biocompatibility |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Mar 2019 07:59 |
| Last Modified: | 25 Mar 2019 07:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3188 |
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