Ferreira-Cerca, Sebastien and Poell, Gisela and Kuehn, Holger and Neueder, Andreas and Jakob, Steffen and Tschochner, Herbert and Milkereit, Philipp (2007) Analysis of the in vivo assembly pathway of eukaryotic 40S ribosomal proteins. MOLECULAR CELL, 28 (3). pp. 446-457. ISSN 1097-2765,
Full text not available from this repository. (Request a copy)Abstract
In eukaryotes, in vivo formation of the two ribosomal subunits from four ribosomal RNAs (rRNAs) and approximately 80 ribosomal proteins (r-proteins) involves more than 150 non-ribosomal proteins and around 100 small non-coding RNAs. It is temporally and spatially organized within different cellular compartments: the nucleolus, the nucleoplasm, and the cytoplasm. Here, we present a way to analyze how eukaryotic r-proteins of the small ribosomal subunit (SSU) assemble in vivo with rRNA. Our results show that key aspects of the assembly of eukaryotic r-proteins into distinct structural parts of the SSU are similar to the in vitro assembly pathway of their prokaryotic counterparts. We observe that the establishment of a stable assembly intermediate of the eukaryotic SSU body, but not of the SSU head, is closely linked to early rRNA processing events. The formation of assembly intermediates of the head controls efficient nuclear export of the SSU and cytoplasmic pre-rRNA maturation steps.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; PRE-RIBOSOMES; CRYSTAL-STRUCTURE; 80S RIBOSOME; SUBUNIT; RNA; YEAST; MATURATION; PROCESSOME; COMPONENTS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Nov 2020 07:20 |
| Last Modified: | 30 Nov 2020 07:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31928 |
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