Moslavac, Suncana and Nicolaisen, Kerstin and Mirus, Oliver and Al Dehni, Fadi and Pernil, Rafael and Flores, Enrique and Maldener, Iris and Schleiff, Enrico (2007) A TolC-Like protein is required for heterocyst development in Anabaena sp strain PCC 7120. JOURNAL OF BACTERIOLOGY, 189 (21). pp. 7887-7895. ISSN 0021-9193, 1098-5530
Full text not available from this repository. (Request a copy)Abstract
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms heterocysts in a semiregular pattern when it is grown on N-2 as the sole nitrogen source. The transition from vegetative cells to heterocysts requires marked metabolic and morphological changes. We show that a trimeric pore-forming outer membrane P-barrel protein belonging to the ToIC family, Alr2887, is up-regulated in developing heterocysts and is essential for diazotrophic growth. Mutants defective in Alr2887 did not form the specific glycolipid layer of the heterocyst cell wall, which is necessary to protect nitrogenase from external oxygen. Comparison of the glycolipid contents of wild-type and mutant cells indicated that the protein is not involved in the synthesis of glycolipids but might instead serve as an exporter for the glycolipid moieties or enzymes involved in glycolipid attachment. We propose that Alr2887, together with an ABC transporter like DevBCA, is part of a protein export system essential for assembly of the heterocyst glycolipid layer. We designate the alr2887 gene hgdD (heterocyst glycolipid deposition protein).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLUE-GREEN-ALGA; NITROGENASE ACTIVITY; MULTIDRUG EFFLUX; OUTER-MEMBRANE; PCC 7120; DIAZOTROPHIC GROWTH; RESPONSE REGULATOR; CRYSTAL-STRUCTURE; CELL-ENVELOPE; CYANOBACTERIUM; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Nov 2020 11:03 |
| Last Modified: | 30 Nov 2020 11:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/31981 |
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